Genetic analysis of MHC Class II <i>DRB</i> gene in an endangered Jamunapari breed of goats

Abstract

Present investigation was aimed to analyze the polymorphism of Class II <i style="">DRB</i> gene region of MHC, using PCR-RFLP with <i style="">Pst</i>I and <i style="">Taq</i>I restriction enzymes, in endangered Jamunapari goat breed. A total of 203 Jamunapari goats (male-77 & female-126) were included in the study to know the distribution of different genotypes in the population. <i style="">Pst</i>I revealed three genotypes, i.e., PP, Pp and pp, with genotype frequency as 0.054, 0.222 and 0.724, respectively. <i style="">Taq</i>I also showed TT, Tt and tt genotypes with frequencies of 0.241, 0.527 and 0.232, respectively. The frequencies of P and p alleles were 0.165 and 0.835, while frequencies of T and t alleles were 0.505 and 0.495, respectively. Marked variations were observed in the distribution of <i style="">Pst</i>I and <i style="">Taq</i>I generated genotypes in the Jamunapari goat population.