A comparative evaluation of cardioprotective activity of two <i>Makandi</i> (<i>Coleus forskohlii</i> Willd.) formulations against isoproterenol induced myocardial infarction in hyperlipidaemic rats

Abstract

The present study was undertaken to evaluate cardioprotective activity of <i>Makandi </i>(<i>Coleus forskohli </i>Willd.) <i>C</i><i style="">hurna</i> and <i>Ghanavati</i> on the basis of electrocardiographic, biochemical, cardiac output and histopathological parameters against isoproterenol (ISO) induced myocardial infarction in diet induced hyperlipidaemic rats. The drug treatment and hyperlipidaemic diets were administered for 20 consecutive days. Myocardial injury was induced by injecting ISO (85 mg/kg) to rats at an interval of 24 h for two days. Forty eight hours after the first dose of ISO injection, parameters like ECG, cardiac output, biochemical and histological observations of the heart tissues were performed. Hyperlipidaemic diet followed by ISO significantly increased heart weight, altered serum lipid profiles, SGPT and ALP activity, caused ST segment elevation, prolonged QT interval and QTc in ECG, increased blood pressure and decreased cardiac output. Histopathologically also, heart showed severe cytoarchitectural disturbances like myonecrosis, fatty changes and endocardial oedema. When administered orally, both the formulations of<i> Makandi</i> decreased atherogenic index, almost normalized ST segment elevation, QT interval prolongation and cardiac output. Histopathologically also remarkable protection was observed. Analysis of the results showed that <i>Makandi</i> in <i>Churna</i> form has both anti-atherogenic potential and cardioprotective activity, while <i>Ghanavti</i> has only weak cardioprotective activity.