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Title: Preferential recognition of methylglyoxal-modified calf thymus DNA by circulating antibodies in cancer patients
Authors: Ahmad, M Imtiaz
Ahmad, Saheem
Keywords: Methylglyoxal;Cancer;Calf thymus DNA;Autoimmunity
Issue Date: Aug-2011
Publisher: NISCAIR-CSIR, India
Abstract: Methylglyoxal (MG) has been implicated in mutagenesis and cancer. Present study probes the antigenicity of MG damaged DNA in cancer patients. Purified calf thymus DNA was damaged by the synergistic action of MG, lysine (Lys) and CuSO4 for 24 h at 37oC. DNA modifications produced single-strand breaks, hyperchromicity in UV spectrum and increased fluorescence intensity. Binding characteristics of auto-antibodies in cancer patients were assessed by direct binding and inhibition ELISA. These antibodies exhibited enhanced binding with the modified DNA, as compared to the native form. The effect was more pronounced when affinity-purified IgG was used in place of the serum. In conclusion, MG-modified DNA presents unique epitopes which are recognized as non-self by the immune system and may, therefore, be one of the factors for the autoantibody induction in cancer patients.
Page(s): 290-296
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.48(4) [August 2011]

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