Homogenate extraction of isoflavones from soybean meal by orthogonal design

Abstract

Factors that affect yield of isoflavones extracted from soybean meal by using homogenate extraction method were studied byorthogonal design experiment [L9(34)]. Optimum extraction conditions were found as follows: sample particle size, 60 mesh;ethanol concentration, 60%; extraction time, 50 s; ratio of material to liquid, 1 g: 20 ml; and yield of isoflavones, 0.52 ± 0.01%.Compared with traditional heat refluxing extraction method, yield of homogenate extraction was 29.3% higher, and extraction timewas significantly shortened.