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http://nopr.niscpr.res.in/handle/123456789/11390| Title: | Comparative study of 6-APA production by free and agar immobilized bacteria in nutrient broth culture |
| Authors: | Dolui, A K Das, S |
| Keywords: | 6- Aminopenicillanic acid;Bioconversion;HPLC assessment;Penicillin G;Penicillin G acylase |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In the present study different bacterial samples were isolated from soil of different places of Dibrugarh and screened for biotransformation ability to produce 6-Aminopenicillanic acid. Among ten isolated bacterial samples, three gram positive bacterial samples designated as AKDD-2, AKDD-4 and AKDD-6 showed the production of 6-APA from penicillin G. Assessment of production of 6-APA after incubation in penicillin G (2 mg/ml) by three different samples separately in free and agar immobilization state was done by HPLC analysis. Reusability of immobilized cells was found successful up to 14 days. |
| Page(s): | 289-292 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.49(04) [April 2011] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 49(4) 289-292.pdf | 382.08 kB | Adobe PDF | View/Open |
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