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Title: | Production and characterization of feather degrading keratinase from Bacillus sp. JB 99 |
Authors: | Kainoor, Pushpalata S Naik, G R |
Keywords: | Bacillus sp. JB 99;feather degradation;feather meal;keratin;keratinase |
Issue Date: | Oct-2010 |
Publisher: | NISCAIR-CSIR, India |
Abstract: | An inducible keratinase is produced by Bacillus sp. JB 99 in a feather medium. Under submerged fermentation condition with continuous agitation (180 rpm), high level of keratinase production occurred at 45oC after 36 h at pH 10. The presence of carbon source in feather medium suppressed the enzyme production, while 0.1% yeast extract enhanced the production. The purified enzyme showed maximum keratinase activity at temperature 65oC and pH 10. The enzyme was monomeric and has a mol wt of approximately 66 kDa (SDS-PAGE). The enzyme may belong to serine protease group as completely inhibited by PMSF. Presence of metal ions, such as, Ca2+, Mg2+, Co2+ and Ba2+ stimulated the enzyme activity, while Hg2+, Pb2+, Zn2+and Fe2+ decreased the activity. Present results indicate that Bacillus sp. JB 99 can be a highly useful organism for feather meal production and in leather industry. |
Page(s): | 384-390 |
ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
Appears in Collections: | IJBT Vol.09(4) [October 2010] |
Files in This Item:
File | Description | Size | Format | |
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IJBT 9(4) 384-390.pdf | 240.69 kB | Adobe PDF | View/Open |
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