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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(08) [August 2010] >
| Title: | Genetic characterization of dengue virus serotypes causing concurrent infection in an outbreak in Ernakulam, Kerala, South India |
| Authors: | Anoop, M
Issac, Aneesh
Mathew, Thomas
Philip, Sairu
Kareem, Nabeel Abdul
Unnikrishnan, R
Sreekumar, E |
| Keywords: | Co-infection
Dengue
Genotyping
Multiplex RT-PCR |
| Issue Date: | Aug-2010 |
| Publisher: | CSIR |
| Abstract: | Dengue fever, a
mosquito-borne viral infection, causes significant morbidity and has become
endemic in the Indian subcontinent. Virus strains currently circulating in many
parts of the country are not well studied at the molecular level. In the
present study, genetic characterization of virus strains from a dengue outbreak
that occurred in and around a tertiary care hospital in Ernakulam, Kerala in the
year 2008 has been reported. By reverse transcription polymerase chain reaction
(RT-PCR), 37 out of 75 (49.3%) clinically suspected cases were positive for dengue
viral RNA. Among these, 21 (56.8%) samples showed concurrent infection with
multiple serotypes of the virus. Majority of the combined infections were
caused by dengue serotype 2 and 3. Co-infections with type 1 and 2 in two
patients, and type 1, 2 and 3 in one patient were also observed. The core-pre-Membrane
(CprM) junction nucleotide sequencing
and phylogenetic analysis revealed that the
type 1 strains were related to the viral strains reported from Delhi-2001 and
Gwalior-2002 dengue outbreaks, while the
type 2 strains were related to the strains from Gwalior-2001 epidemic. Sequences
of type 3 strains did not show clear relation to any of the previous Indian
isolates, and in the phylogenetic analysis, they formed a distinct lineage
within the Indian type 3 strains. This study indicates hyperendemicity of dengue
in the region with the presence of multiple serotypes and high rates of
co-infection, and local genomic evolution of the viral strains involved in this
outbreak. |
| Page(s): | 849-857 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(08) [August 2010]
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