20-Jun-2013
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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(08) [August 2010] >
| Title: | Antinociceptive and acute toxicity evaluation of Vernonia condensata Baker leaves extracted with different solvents and their mixtures |
| Authors: | Risso, Wagner E
Scarminio, Ieda S
Moreira, Estefania G |
| Keywords: | Analgesic
Antiinflammatory
Lethal dose
Phytochemical screening
Vernonia condensata
Writhing test |
| Issue Date: | Aug-2010 |
| Publisher: | CSIR |
| Abstract: | Extract of Vernonia condensata (Asteraceae =
Compositae) leaves has different uses in Brazilian folk medicine, which
includes analgesic and antiinflamatory agent. The aim of this study was to
apply a modified simplex-centroid mixture design to evaluate the best extractor
system for the antinociceptive activity, evaluated by writhing test. Different
solvents (acetone, dichloromethane, ethanol and ethyl acetate) as well as their
binary, ternary and quaternary mixtures were used. For comparison, aqueous
extract was also evaluated. LD50 was estimated and qualitative
phytochemical screening, conducted. The extracts with antinociceptive activity
were: aqueous, acetone, dicloromethane (DCM), ethanol (ETOH), acetone-DCM,
acetone-ETOH, acetone-ethyl acetate, ETOH-ethyl acetate, acetone-DCM-ethyl
acetate, acetone-ETOH-ethyl acetate and DCM-ETOH-ethyl acetate. The higher
margin of safety (LD50/ED50) was for acetone >
acetone-ETOH-ethyl acetate > aqueous > ETOH = acetone-ETOH > DCM >
acetone-ethyl acetate > DCM-ETOH-ethyl acetate > acetone-DCM >
acetone-DCM-ethyl acetate. Phytochemical screening showed that all the extracts
contained alkaloids, phenolic compounds, flavonoids, tannins and saponins. In
conclusion, the extractor system influences both the pharmacological activity
and acute toxicity of leaves from V.
condensata. Acetone and the ternary mixture, acetone-ETOH-ethyl acetate
extracts showed higher margin of safety than aqueous extract. |
| Page(s): | 811-816 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(08) [August 2010]
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