Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/9985
Title: Antidiabetic effect of <i style="">Dodonaea viscosa </i>(L). Lacq. aerial parts in high fructose-fed insulin resistant rats: A mechanism based study
Authors: Veerapur, V P
Prabhakar, K R
Thippeswamy, B S
Bansal, Punit
Srinivasan, K K
Unnikrishnan, M K
Keywords: <b style=""> </b><i style="">Dodonaea viscosa</i>
DPP-IV
Homeostatic model assessment
HPLC
PPAR-<img src='/image/spc_char/gamma.gif' border=0>
Prediabetic
PTP-1B
Quercetin
Issue Date: Aug-2010
Publisher: CSIR
Abstract: To study the effect and mode of action of water extract (DVW) and polar fraction of ethanol extract (DVE-4) of <i style="">D. viscosa </i>in high-fructose diet induced insulin resistance in male Wistar rats. <i style="">D. viscosa’</i>s<i style=""> </i>effects were evaluated on a battery of targets involved in glucose homeostasis (<i style="">in vitro</i> studies). Rats were rendered insulin resistant by feeding 66% (w/w) fructose and 1.1% (v/w) coconut oil mixed with normal pellet diet (NPD) for six weeks. DVW and DVE4 at different doses were administered simultaneously. At the end of the study, blood glucose, oral glucose tolerance test, lipid profile and insulin were estimated and homeostatic model assessment (HOMA) levels were calculated. In addition, enzymatic and non-enzymatic liver antioxidant levels were also estimated. Quantification of biomarker quercetin was done using HPLC. Fructose diet with DVW, DVE-4 significantly reduced blood glucose, serum insulin, HOMA, lipid profiles and significantly improved glucose tolerance and HDL-c levels. In addition, these extract and fraction also decreased oxidative stress by improving endogenous antioxidants. In different bioassays, DVW and DVE-4 inhibited protein tyrosine phosphatase-1B with IC<sub>50</sub> 65.8 and 54.9 <img src='/image/spc_char/micro.gif' border=0> g/ml respectively and showed partial inhibition of dipeptidyl peptidase-IV. Moreover, DVW and DVE-4, at 10 mg/ml showed 60 and 54.2% binding to peroxisome proliferator-activated receptor-g. Further, 2.1% (w/w) of quercetin was quantified in bioactive-DVE-4 using HPLC method. The results provide pharmacological evidence of <i style="">D. viscosa</i> in treatment of prediabetic conditions and these effects may be mediated by interacting with multiple targets operating in diabetes mellitus.
Description: 800-810
URI: http://hdl.handle.net/123456789/9985
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.48(08) [August 2010]

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