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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(08) [August 2010] >
| Title: | Macrophage apoptosis associated with Salmonella enterica serovar Typhi plasmid |
| Authors: | Wu, Shuyan
Li, Yuanyuan
Xu, Yang
Song, Guorong
Qin, Zhenghong
Huang, Rui |
| Keywords: | Salmonella enterica serovar Typhi, Plasmid
Macrophage
Apoptosis |
| Issue Date: | Aug-2010 |
| Publisher: | CSIR |
| Abstract: | The present study was undertaken to
investigate the relationship between plasmid isolated from S. enterica serovar
Typhi (pRST98) and macrophage apoptosis. pRST98 was
transferred into an attenuated S. enterica serovar
Typhimurium strain RIA to create a transconjugant pRST98/RIA.
Standard S. enterica serovar
Typhimurium virulence strain SR-11 was used as a positive control, and RIA as a negative
one. Murine macrophage-like cell line (J774A.1) was used as an infectious cell
model in vitro. In order to determine the
inhibition and bactericidal effect of amikacin (AMK) to extracellular
bacteria and the best optimization co-culture ratio between Salmonella and J774A.1, the
minimal inhibitory concentration (MIC) and minimal bactericidal concentration
(MBC) of AMK to strains SR-11, pRST98/RIA and RIA and multiplicity
of infection (MOI) were detected
first, and then J774A.1 was infected by the above three
serovar Typhimurium strains. Apoptosis of J774A.1 was
examined with electron microscopy and flow cytometry after annexin-V/propidium iodide
labeling at 0, 1, 3, 6, 12 and 24 h. Mitochondrial membrane potential was detected
by JC-1 staining method. It was demonstrated that MIC of AMK to the three
strains was 10µg/ml, MBC was 80µg/ml, and optimal MOI was 100:1. pRST98/RIA
resulted in a higher
apoptosis of J774A.1 than RIA, apoptotic features such
as chromatin margination could be observed after 3 h, and death of J774A.1 cells
was associated with the loss of mitochondrial membrane potential.
These results indicated that pRST98 could enhance the virulence of
its host bacteria, evidenced by increased macrophage apoptosis. |
| Page(s): | 773-777 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(08) [August 2010]
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