NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.44 [2007] >
IJBB Vol.44(2) [April 2007] >

Title: Studies on interactions between plant secondary metabolites and glutathione transferase using fluorescence quenching method
Authors: Zhang, Xian
Cheng, Xinsheng
Wang, Chuanqin
Xue, Zechun
Yang, Liwen
Xi, Zheng
Keywords: Plant secondary metabolites
Glutathione transferase
Fluorescence quenching
UV-Vis absorption spectroscopy
Issue Date: Apr-2007
Publisher: CSIR
Abstract: The interactions between plant secondary metabolites (tannic acid, rutin, cinnamic acid and catechin) and glutathione transferase (GST) were investigated by fluorescence and UV-Vis absorption spectroscopy. Intrinsic fluorescence of GST was measured by selectively exciting their tryptophan (Trp) residues and quenching constants were determined using the Stern-Volmer equation. The binding affinity was found to be strongest for tannic acid and ranked in the order tannic acid>rutin>cinnamic acid>catechin. The pH values in the range of 6.7-7.9, except for tannic acid, did not affect significantly the affinity of rutin, cinnamic acid and catechin with GST. Results showed that the fluorescence quenching of GST was a static quenching. Fluorescence quenching and UV-Vis absorption spectroscopy suggested that only the tannic acid changed the microenvironment of the Trp residues. Furthermore, the number of binding sites and binding constants at different pH values showed that tannic acid had strongest affinity towards GST and hydrogen bonding played an important role in the affinity between GST and the metabolites.
Page(s): 101-105
ISSN: 0301-1208
Source:IJBB Vol.44(2) [April 2007]

Files in This Item:

File Description SizeFormat
IJBB 44(2) (2007) 101-105.pdf278.8 kBAdobe PDFView/Open
 Current Page Visits: 1101 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171902 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: