Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/9895
Title: Direct organogenesis from hypocotyl explants of <i style="">Psoralea corylifolia </i>L.—An endangered medicinal plant
Authors: Baskaran, P
Jayabalan, N
Keywords: Hypocotyl
plant regeneration
<i style="">Psoralea corylifolia</i>
Issue Date: Jul-2010
Publisher: CSIR
Abstract: A protocol has been developed for <i style="">in vitro </i>regeneration of <i style="">Psoralea corylifolia </i>L. through adventitious shoot regeneration from hypocotyl explants cultured on Murashige and Skoog (MS) medium supplemented with various combinations and concentrations of plant growth regulators. The highest response was obtained on MS medium containing 3.0 µ<i style="">M</i> benzyladenine (BA), 1.0 µ<i style="">M</i> naphthalene acetic acid (NAA), 5.0 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> ascorbic acid (AA) and 100 mg L<sup>-1 </sup>casein hydrolysate (CH). The regenerated shoots were rooted on half-strength MS basal medium supplemented with 3.0 µ<i style="">M</i> indole-3-butyric acid (IBA). Rooted shoots were effectively transferred to garden soil, farmyard soil and sand (2:1:1 ratio) mixture. Subsequently, the plants were successfully acclimatized. During acclimatization, the plants were irrigated with 50 mL one-eighth strength MS basal salt solution devoid of sucrose and inositol, but supplemented with 3.0 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> IBA and 100 mg L<sup>–1</sup> bavistin (BVN). The plants were subsequently established in the field.
Description: 329-332
URI: http://hdl.handle.net/123456789/9895
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(3) [July 2010]

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