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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(3) [July 2010] >
| Title: | Direct organogenesis from hypocotyl explants of Psoralea corylifolia L.—An endangered medicinal plant |
| Authors: | Baskaran, P
Jayabalan, N |
| Keywords: | Hypocotyl
plant regeneration
Psoralea corylifolia |
| Issue Date: | Jul-2010 |
| Publisher: | CSIR |
| Abstract: | A protocol has been
developed for in vitro regeneration
of Psoralea corylifolia L. through
adventitious shoot regeneration from hypocotyl explants cultured on Murashige
and Skoog (MS) medium supplemented with various combinations and concentrations
of plant growth regulators. The highest response was obtained on MS medium
containing 3.0 µM benzyladenine (BA),
1.0 µM naphthalene acetic acid (NAA),
5.0  M ascorbic acid (AA) and 100 mg L-1
casein hydrolysate (CH). The regenerated shoots were rooted on
half-strength MS basal medium supplemented with 3.0 µM indole-3-butyric acid (IBA). Rooted shoots were effectively
transferred to garden soil, farmyard soil and sand (2:1:1 ratio) mixture.
Subsequently, the plants were successfully acclimatized. During
acclimatization, the plants were irrigated with 50 mL one-eighth strength MS
basal salt solution devoid of sucrose and inositol, but supplemented with 3.0 M IBA and 100 mg L–1 bavistin
(BVN). The plants were subsequently established in the field. |
| Page(s): | 329-332 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(3) [July 2010]
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