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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(3) [July 2010] >
| Title: | Intraspecific variation in the internal transcribed spacer (ITS) regions of rDNA in Withania somnifera (Linn.) Dunal |
| Authors: | Mir, Bilal Ahmad
Koul, Sushma
Kumar, Arun
Kaul, Maharaj K
Soodan, Amarjit Singh
Raina, Soom Nath |
| Keywords: | Withania somnifera
RFLP
internal transcribed spacer (ITS)
intraspecific variation |
| Issue Date: | Jul-2010 |
| Publisher: | CSIR |
| Abstract: | Intraspecific variation in ITS regions of
the rDNA among the five wild and five cultivated genotypes of Withania somnifera, were evaluated at nucleotide sequence level
using restriction
fragment length polymorphism (RFLP). The entire internal transcribed spacer
(ITS1-5.8S-ITS2) region was first amplified by PCR and then cleaved with four
different restriction enzymes (EcoRV, Hinf I, Afa I & Hae III). Restriction endonuclease
digests, types, and sequence length composition of ITS 1 and ITS 2 of nuclear
ribosomal DNA provided discrete differences
between the cultivated and wild genotypes. A 710 bp single
amplified product was obtained in all the five wild genotypes whereas, two ITS
bands named as ITS type A and B of 709 bp and 552 bp, respectively were
obtained in the five cultivated genotypes. A single deletion at 672 position
was noted in ITS type A of cultivated genotypes. There was no restriction site
in 552 bp ITS band for all the four restriction enzymes used. The variation of
ITS at amplification as well as digestion level is in conformity with morphological
and phytochemical differences in
W. somnifera genotypes. |
| Page(s): | 325-328 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(3) [July 2010]
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