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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(3) [July 2010] >
| Title: | Plant regeneration from alginate-encapsulated somatic embryos of Dalbergia sissoo Roxb. |
| Authors: | Singh, Ajay Kumar
Chand, Suresh |
| Keywords: | Dalbergia sissoo
encapsulation
legume tree
somatic embryogenesis
synthetic seeds |
| Issue Date: | Jul-2010 |
| Publisher: | CSIR |
| Abstract: | A method has been developed for plant
regeneration by encapsulation of somatic embryos obtained from callus cultures
derived from semi-mature cotyledon explants of Dalbergia sissoo Roxb. (family Fabaceae). Embryogenic callus was developed
from cotyledon pieces on Murashige and Skoog (1962) medium supplemented with
9.04 mM 2,4-dichlorophenoxyacetic acid and 0.46 mM kinetin. The somatic embryos were induced from embryogenic callus
on hormone free ½-MS medium with 2% sucrose. Cotyledonary stage somatic embryos were encapsulated using sodium
alginate (2.5%) and calcium chloride (75 mM)
as gelling matrix. The highest frequency (43.3%) for
conversion of encapsulated somatic embryos into plantlets was achieved on ½-MS
medium with 2% sucrose. Plantlets with well developed shoots and roots were established
in pots containing autoclaved mixture of peat moss and soil. Under ex vitro conditions, the conversion of
encapsulated somatic embryos into plantlets was also achieved when these were directly
sown in autoclaved peat moss moistened with ½-MS0 medium. |
| Page(s): | 319-324 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(3) [July 2010]
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