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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(3) [July 2010] >
| Title: | Micropropagation of Verbesina encelioides—An invasive weed |
| Authors: | Jain, Satish C
Jain, Renuka
Singh, Renu |
| Keywords: | :Callusing
micropropagation
plant regeneration
Verbesina encelioides |
| Issue Date: | Jul-2010 |
| Publisher: | CSIR |
| Abstract: | An efficient protocol for in vitro micropropagation of
V. encelioides (Cav.) Benth. & Hook. fil. ex Gray, a medicinal weed, through hypocotyl
segments of seedlings was developed on Murashige and Skoog’s (MS) medium
supplemented with various concentrations of α-naphthalene acetic acid (NAA,
0.50-15.00 mg/L), 6-benzylaminopurine (BAP, 1.00-3.00
mg/L) and kinetin
(Kn, 0.20-0.60 mg/L). Highly organized callus induction and
proliferation were achieved on MS medium+NAA
(1.00 mg/L)+BAP (3.00 mg/L). Optimum shoot multiplication (13.00±0.28) was
achieved on MS medium+NAA
(0.50 mg/L)+BAP (1.00 mg/L) with the shoot height of 1.80±
0.15 cm. Similarly, efficient root induction (8.30±0.40) was achieved on MS
medium+indole 3-acetic acid (IAA, 1.00 mg/L) with the root length of 2.60± 0.34
cm as 83% of the microshoots produced roots. These plantlets were hardened in a
controlled plant growth chamber, prior to ex-vitro
transfer. No phenotypic differences were observed among the regenerates. |
| Page(s): | 333-335 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(3) [July 2010]
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