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Title: Micropropagation of Verbesina encelioides—An invasive weed
Authors: Jain, Satish C
Jain, Renuka
Singh, Renu
Keywords: :Callusing
plant regeneration
Verbesina encelioides
Issue Date: Jul-2010
Publisher: CSIR
Abstract: An efficient protocol for in vitro micropropagation of V. encelioides (Cav.) Benth. & Hook. fil. ex Gray, a medicinal weed, through hypocotyl segments of seedlings was developed on Murashige and Skoog’s (MS) medium supplemented with various concentrations of α-naphthalene acetic acid (NAA, 0.50-15.00 mg/L), 6-benzylaminopurine (BAP, 1.00-3.00 mg/L) and kinetin (Kn, 0.20-0.60 mg/L). Highly organized callus induction and proliferation were achieved on MS medium+NAA (1.00 mg/L)+BAP (3.00 mg/L). Optimum shoot multiplication (13.00±0.28) was achieved on MS medium+NAA (0.50 mg/L)+BAP (1.00 mg/L) with the shoot height of 1.80± 0.15 cm. Similarly, efficient root induction (8.30±0.40) was achieved on MS medium+indole 3-acetic acid (IAA, 1.00 mg/L) with the root length of 2.60± 0.34 cm as 83% of the microshoots produced roots. These plantlets were hardened in a controlled plant growth chamber, prior to ex-vitro transfer. No phenotypic differences were observed among the regenerates.
Page(s): 333-335
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(3) [July 2010]

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