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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(3) [July 2010] >
| Title: | Biotization with Piriformospora indica and Pseudomonas fluorescens improves survival rate, nutrient acquisition, field performance and saponin content of micropropagated Chlorophytum sp. |
| Authors: | Gosal, S K
Karlupia, A
Gosal, S S
Chhibba, I M
Varma, A |
| Keywords: | Chlorophytum
micropropagation
micronutrient
Piriformospora indica
Pseudomonas fluorescens
saponin |
| Issue Date: | Jul-2010 |
| Publisher: | CSIR |
| Abstract: | Micropropagated
plantlets usually exhibit high mortality rate upon their transfer to soil as a
result of transplantation shock caused by abiotic and biotic stresses and weak
root system in the absence of beneficial microflora. Biotization of
micropropagated Chlorophytum sp. with the fungus, Piriformospora indica and the bacterium, Pseudomonas fluorescens, improved plantlet survival rate, growth
parameters, field performance, P content and the micronutrient acquisition.
Biotized plants showed root colonization of P. indica in cortical cells
of roots and exhibited the presence of pear shaped spores and hyphae as well as
rhizospheric colonization of P. fluorescens. Microbial biotization
enhanced plant survival up to 91.2% by dual inoculation over uninoculated
control (78.8%), on transfer from laboratory to green house. Biotized field
grown plants exhibited increase in root length, number of lateral roots, shoot
dry weight, leaf length, number and dry weight of fleshy roots in dual
inoculation at PF which were significantly better over single as
well as uninoculated control. Plants inoculated with P. indica exhibited
maximum chlorophyll content (8.76 mg g-1)
while maximum P content (0.26%) was observed in dual inoculated plants, which
was at par with P. indica alone even at low phosphorus. Higher
saponin content was observed with both, P. indica alone as well as dual
inoculations. Maximum acquisition of Cu (40 μg g-1) was observed in P. indica inoculated plants at PF level, which was at
par with dual inoculation. In dual inoculated plants, the highest contents of
Fe (4405 μg g-1),
Zn (135 μg g-1)
and Mn (160 μg g-1)
were observed. Thus, microbial biotization improved survival and nutrient
uptake by micropropagated Chlorophytum plants. |
| Page(s): | 289-297 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(3) [July 2010]
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