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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(3) [July 2010] >
| Title: | Evaluation of genetic diversity among Jatropha curcas (L.) by RAPD analysis |
| Authors: | Subramanyam, K
Rao, Dowlathabad Muralidhara
Devanna, N
Aravinda, A
Pandurangadu, V |
| Keywords: | Cluster analysis
DNA fingerprinting
genetic diversity
Jatropha curcas
PCR
principal component analysis |
| Issue Date: | Jul-2010 |
| Publisher: | CSIR |
| Abstract: |
The genetic diversity and pedigree analysis of Jatropha curcas (L.) was evaluated through RAPD markers. A total number of 10
accessions, collected from different ecoclimatic zones of India, were
screened with 43 random decamer primers to evaluate polymorphism. Selected 10
primers generated 125 bands, 76 of which were found to be polymorphic. The
amplification products ranged from 9-14 bands for different primers. Each
primer produced on an average 12.5 bands per primer, of which 7.6 were
polymorphic. UPGMA method was used to construct dendrogram and genetic distance
matrix was determined using squared Euclidean distances. Cluster analysis of
data using UPGMA algorithm placed the ten
accessions into two main clusters,
the genetic dissimilarity matrix between genotypes ranged from 0-8 and the principal
component analysis placed the 10 accessions into three groups.
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| Page(s): | 283-288 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(3) [July 2010]
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