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IJBT Vol.09(3) [July 2010] >


Title: Evaluation of genetic diversity among Jatropha curcas (L.) by RAPD analysis
Authors: Subramanyam, K
Rao, Dowlathabad Muralidhara
Devanna, N
Aravinda, A
Pandurangadu, V
Keywords: Cluster analysis
DNA fingerprinting
genetic diversity
Jatropha curcas
PCR
principal component analysis
Issue Date: Jul-2010
Publisher: CSIR
Abstract:  The genetic diversity and pedigree analysis of Jatropha curcas (L.) was evaluated through RAPD markers. A total number of 10 accessions, collected from different ecoclimatic zones of India, were screened with 43 random decamer primers to evaluate polymorphism. Selected 10 primers generated 125 bands, 76 of which were found to be polymorphic. The amplification products ranged from 9-14 bands for different primers. Each primer produced on an average 12.5 bands per primer, of which 7.6 were polymorphic. UPGMA method was used to construct dendrogram and genetic distance matrix was determined using squared Euclidean distances. Cluster analysis of data using UPGMA algorithm placed the ten accessions into two main clusters, the genetic dissimilarity matrix between genotypes ranged from 0-8 and the principal component analysis placed the 10 accessions into three groups.
Page(s): 283-288
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(3) [July 2010]

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