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Title: RAPD analysis in mungbean [<i style="">Vigna radiata</i> (L.) Wilczek.] II: A comparison of efficiency parameters of RAPD primers
Authors: Saini, Madhu
Singh, Subhadra
Hussain, Z
Sikka, V K
Keywords: Discriminatory power
diversity index
marker index
rank correlation
RAPD pattern
resolving power
Issue Date: Jul-2010
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"> The study was undertaken to identify the efficient RAPD primers that could differentiate a set of 39 mungbean [<i style="">Vigna radiata</i> (L.) Wilczek] genotypes. Various efficiency parameters, namely, discriminatory power (D), discriminatory power with infinite sample size (D<sub>L</sub>), diversity index (DI), resolving power (R<sub>p</sub>) and marker index (MI) were studied for 30 primers. The relationship between the parameters was studied using Spearman rank correlation coefficient. The parameters D and D<sub>L</sub> were the most efficient, followed by R<sub>p</sub>, MI and DI. DI was not a good parameter in those cases where a set of primers with intermediate range of variation in their efficiency were used. None of the 30 primers alone could differentiate all 39 mungbean genotypes. However, a methodology could be designed to find the primer/primers combination to differentiate all 39 genotypes. The primers S-1 and S-2 were more efficient with iso-frequency distribution of most of their banding patterns and a combination of any one of the primers with S-1 or S-2 could identify all the genotypes. The study showed how to select the minimum number of efficient primers aimed to distinguish all genotypes cost-effectively. </smarttagtype></smarttagtype></smarttagtype>
Description: 276-282
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(3) [July 2010]

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