NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(3) [July 2010] >


Title: RAPD analysis in mungbean [Vigna radiata (L.) Wilczek.] II: A comparison of efficiency parameters of RAPD primers
Authors: Saini, Madhu
Singh, Subhadra
Hussain, Z
Sikka, V K
Keywords: Discriminatory power
diversity index
marker index
mungbean
rank correlation
RAPD pattern
resolving power
Issue Date: Jul-2010
Publisher: CSIR
Abstract:  The study was undertaken to identify the efficient RAPD primers that could differentiate a set of 39 mungbean [Vigna radiata (L.) Wilczek] genotypes. Various efficiency parameters, namely, discriminatory power (D), discriminatory power with infinite sample size (DL), diversity index (DI), resolving power (Rp) and marker index (MI) were studied for 30 primers. The relationship between the parameters was studied using Spearman rank correlation coefficient. The parameters D and DL were the most efficient, followed by Rp, MI and DI. DI was not a good parameter in those cases where a set of primers with intermediate range of variation in their efficiency were used. None of the 30 primers alone could differentiate all 39 mungbean genotypes. However, a methodology could be designed to find the primer/primers combination to differentiate all 39 genotypes. The primers S-1 and S-2 were more efficient with iso-frequency distribution of most of their banding patterns and a combination of any one of the primers with S-1 or S-2 could identify all the genotypes. The study showed how to select the minimum number of efficient primers aimed to distinguish all genotypes cost-effectively.
Page(s): 276-282
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(3) [July 2010]

Files in This Item:

File Description SizeFormat
IJBT 9(3) 276-282.pdf558.28 kBAdobe PDFView/Open
 Current Page Visits: 765 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 570282 since 06-Feb-2009  Last updated on 14-Aug-2014Webmaster: nopr@niscair.res.in