Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/9832
Title: Micropropagation of <i style="">Tribulus terrestris</i> Linn
Authors: Raghu, A V
Martin, Gerald
Balachandran, Indira
Mohanan, K V
Keywords: Direct shoot regeneration
Medicinal plant
Micropropagation
Nodal explants
Tissue culture
<i>Tribulus terrestris</i>
Woody Plant Medium
Issue Date: Jun-2010
Publisher: CSIR
Series/Report no.: <b style="">Int. cl.<sup>8</sup> </b><b style="">¾ </b>A61K 36/00, A01G 7/00, A01G 31/00<b></b>
Abstract: A simple and efficient method for high frequency direct shoot regeneration from nodal explants of <i>Tribulus terrestris</i> Linn. (Family ¾ Zygophyllaceae), a medicinally important plant is described in this paper. Woody Plant Medium (WPM) supplemented with 4.0 mg/l<sup> </sup>BA was found to be most effective in inducing bud break, growth and also in initiating multiple shoot proliferation at the rate of six to seven micro-shoots per nodal explant after four weeks of culture. A high frequency multiplication rate was established by repeated sub-culturing. Excised shoots were rooted on MS basal medium without any growth regulators and got established after a hardening process with 80% survival. Micropropagated plants grew well under field conditions, attained maturity and flowered. No phenotypical differences were observed among regenerated plants.
Description: 232-235
URI: http://hdl.handle.net/123456789/9832
Appears in Collections:IJNPR Vol.1(2) [June 2010]

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