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IJNPR Vol.1(2) [June 2010] >


Title: Micropropagation of Tribulus terrestris Linn
Authors: Raghu, A V
Martin, Gerald
Balachandran, Indira
Mohanan, K V
Keywords: Direct shoot regeneration
Medicinal plant
Micropropagation
Nodal explants
Tissue culture
Tribulus terrestris
Woody Plant Medium
Issue Date: Jun-2010
Publisher: CSIR
IPC CodeInt. cl.8 ¾ A61K 36/00, A01G 7/00, A01G 31/00
Abstract: A simple and efficient method for high frequency direct shoot regeneration from nodal explants of Tribulus terrestris Linn. (Family ¾ Zygophyllaceae), a medicinally important plant is described in this paper. Woody Plant Medium (WPM) supplemented with 4.0 mg/l BA was found to be most effective in inducing bud break, growth and also in initiating multiple shoot proliferation at the rate of six to seven micro-shoots per nodal explant after four weeks of culture. A high frequency multiplication rate was established by repeated sub-culturing. Excised shoots were rooted on MS basal medium without any growth regulators and got established after a hardening process with 80% survival. Micropropagated plants grew well under field conditions, attained maturity and flowered. No phenotypical differences were observed among regenerated plants.
Page(s): 232-235
Source:IJNPR Vol.1(2) [June 2010]

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