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Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
IJNPR Vol.1 [2010] >
IJNPR Vol.1(2) [June 2010] >
| Title: | Antibacterial activity of black pepper (Piper nigrum Linn.) with special reference to its mode of action on bacteria |
| Authors: | Karsha, Pavithra Vani
Lakshmi, O Bhagya |
| Keywords: | Antibacterial activity
Black pepper
Piper nigrum
Gram positive bacteria
Gram negative bacteria
Nucleic acids |
| Issue Date: | Jun-2010 |
| Publisher: | CSIR |
| IPC Code: | Int. cl.8 ¾ A61K 36/00, A61K 36/67, A61K 131/00, A61P 31/00, A61P 31/04 |
| Abstract: | During present study the antibacterial activity of black pepper (Piper nigrum Linn.) and its mode of action on bacteria were done. The
extracts of black pepper were evaluated for antibacterial activity by disc
diffusion method. The minimum inhibitory concentration (MIC) was determined by
tube dilution method and mode of action was studied on membrane leakage of UV260
and UV280 absorbing material spectrophotometrically.
The diameter of the zone of inhibition
against various Gram positive and Gram negative bacteria was measured. The MIC
was found to be 50-500ppm. Black pepper altered the membrane permeability
resulting the leakage of the UV260 and UV280 absorbing
material i.e., nucleic acids and proteins into the extra cellular medium. The
results indicate excellent inhibition on the growth of Gram positive bacteria
like Staphylococcus aureus, followed
by Bacillus cereus and Streptococcus faecalis. Among the Gram
negative bacteria Pseudomonas aeruginosa
was more susceptible followed by Salmonella
typhi and Escherichia coli. |
| Page(s): | 213-215 |
| Source: | IJNPR Vol.1(2) [June 2010]
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