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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(3) [June 2010] >
| Title: | Regulation of growth and antioxidant enzyme activities by 28-homobrassinolide in seedlings of Raphanus sativus L. under cadmium stress |
| Authors: | Sharma, Indu
Pati, Pratap Kumar
Bhardwaj, Renu |
| Keywords: | 28-Homobrassinolide
Antioxidant enzymes
Raphanus sativus
Cd metal stress |
| Issue Date: | Jun-2010 |
| Publisher: | CSIR |
| Abstract: | 28-Homobrassinolide
(28-HBL), a brassinosteroid is reported to play significant role in diverse
physiological processes. It induces a range of cellular and adaptive responses
to a range of environmental stresses. Cadmium (Cd) is a non-essential metal which
alters various physiological processes and generates ROS, which can oxidize
biological macromolecules and causes oxidative stress. This
stress is generally overcome by the internal antioxidative defense system and
stress shielding phytohormones. In this study, effect of 28-HBL was studied on growth and activities of antioxidant enzymes in
known hyperaccumulator Raphanus
sativus L. (radish)
seedlings grown under cadmium (Cd) metal stress. To
determine the influence of 28-HBL (0, 10-11,
10-9, 10-7 M) in radish seedlings subjected to Cd (0,
0.5, 1.0, 1.5 mM) stress, the activities of antioxidant enzymes
(APOX, CAT, GR, POD and SOD) were analyzed. In
addition, length and biomass of radish seedlings was also recorded. Cd toxicity
resulted in reduced length, biomass, protein content and activities of
antioxidant enzymes. 28-HBL treatments lowered the Cd toxicity by enhancing the activities of antioxidant
enzymes, biomass and seedling length. The present study thus suggests a
possible role of 28-HBL in amelioration of metal stress by regulating the
activities of antioxidant enzymes in radish. |
| Page(s): | 172-177 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(3) [June 2010]
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