|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(3) [June 2010] >
| Title: | Evaluation of suitable solvents for testing the anti-proliferative activity of triclosan - a hydrophobic drug in cell culture |
| Authors: | Vandhana, S
Deepa, P R
Aparna, G
Jayanthi, U
Krishnakumar, S |
| Keywords: | Triclosan
Cytotoxicity
Cell culture
Drug sensitivity
Acetone
Polyethylene glycol |
| Issue Date: | Jun-2010 |
| Publisher: | CSIR |
| Abstract: | Triclosan, a broad spectrum antibiotic is
currently being evaluated for its anti-cancer property. Though several solvents
are available to dissolve lipophilic (hydrophobic) drugs, solubility and
toxicity aspects pose a challenge, when combined with the cell culture medium.
In this paper, we present a simple approach based on physico-chemical and
biologic criteria to choose a suitable solubilizing agent to study the
anti-proliferative property of triclosan in breast cancer cell line MCF-7.
Triclosan was dissolved in five different solvents viz. DMSO, absolute ethanol,
1 N NaOH, 55% polyethylene glycol + 45% ethanol mixture
(PEM) and acetone and diluted with the culture medium
(1 mg/ml). Although triclosan dissolved completely in all five solvents, on
dilution with culture medium, turbidity was observed in DMSO, 1 N NaOH and
ethanol. Cell viability was 95.23% in 10 ml of acetone, when compared with 49.45% at the same volume of PEM.
This non-toxic nature of acetone was supported by DNA fragmentation analysis
and phase contrast microscopy. A significant decrease in cancer cell
proliferation at 100 mg/ml of
acetone-solubilized triclosan, compared with 100 mg/ml of PEM-solubilized triclosan (p<0.05) indicated stronger
anti-proliferative effect and greater drug-sensitivity of triclosan when solubilized
in acetone. Results showed that acetone-solubilized triclosan was suitable for anti-cancer
investigations in cultured MCF-7 cells. |
| Page(s): | 166-171 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(3) [June 2010]
|
|
