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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(3) [June 2010] >
| Title: | DNA damage by ribose: Inhibition at high ribose concentrations |
| Authors: | Waris, Sahar
Pischetsrieder, Monika
Saleemuddin, Mohammed |
| Keywords: | Ribose
DNA glycation
DNA-AGEs |
| Issue Date: | Jun-2010 |
| Publisher: | CSIR |
| Abstract: | Nucleobases and DNA react non-enzymatically
with sugars, and generate DNA-advanced glycation end products (DNA-AGEs).
Incubation of plasmid pBr322 with ribose for 3-7 days caused the transformation
of the supercoiled plasmid to the open circular and linear forms. Removal of
sugar after an initial 24 h incubation resulted in marked enhancement in the transformation
rate. Enhancement in transformation was also observed when bovine serum albumin
(BSA) exposed to ribose for short durations was incubated with plasmid in
absence of the sugar. The effect on DNA was attenuated when excess ribose
remained in the incubation mixture of ribose and protein. The data suggested
that an increase in ribose concentration in the vicinity of DNA could be
damaging and the damage exacerbated, if sugar levels fell subsequently.
Incubation of herring sperm DNA with ribose resulted in a concentration and
time-dependent increase of N2-carboxyethyl-2’-deoxyguanosine (CEdGA,B).
The concentration of CEdGA,B, however, did not increase further when
ribose was removed from the reaction mixture. |
| Page(s): | 148-156 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(3) [June 2010]
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