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Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(3) [June 2010] >
| Title: | In vitro studies on inhibitory effect of proanthocyanidins in modulation of neutrophils and macrophages |
| Authors: | Govindaraj, Jayamathi
Emmadi, Pamela
Puvanakrishnan, Rengarajulu |
| Keywords: | Lysosomal enzymes
Macrophages
Neutrophils
Proanthocyanidins
Reactive nitrogen species
Reactive oxygen species |
| Issue Date: | Jun-2010 |
| Publisher: | CSIR |
| Abstract: | The role of proanthocyanidins (PC), a novel
flavonoid extracted from grape seeds was studied in vitro in the
modulation of neutrophil and macrophage function. We attempted to assess the
levels of non-enzymatic and enzymatic mediators in the presence or absence of
PC in 4-phorbol-12- -myristate-13-acetate (PMA)-stimulated
neutrophils isolated from humans and rats, E. coli endotoxin-stimulated
macrophages and macrophages isolated from E. coli
endotoxin-induced experimental periodontitis in rats. Addition of PC at
a concentration of 50 µg/ml effectively blocked the release of reactive oxygen
species (ROS) and reactive nitrogen species (RNS) and exhibited a marked
inhibition of myeloperoxidase (MPO) and lysosomal enzymes (p<0.001), as compared to PMA-stimulated
neutrophils (human and rats) and neutrophils isolated from experimental
periodontitis in rats. The levels of ROS, RNS and lysosomal enzymes were found to be elevated (p<0.001) and addition of PC
significantly (p<0.001) reduced these levels as compared to those from E. coli
endotoxin-stimulatedmacrophages from rats and macrophages
isolated from experimental periodontitis in rats (p<0.001).
Thus, the study demonstrated that PC decreased the levels of ROS and RNS and also inhibited the MPO and lysosomal enzymes activities in experimental periodontitis in rats. In addition, this study clearly indicated that PC
could be developed as an effective antiinflammatory agent. |
| Page(s): | 141-147 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(3) [June 2010]
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