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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(07) [July 2010] >
| Title: | Acute and sub-acute toxicity of an insect pheromone, N-heneicosane and combination with insect growth regulator, diflubenzuron, for establishing no observed adverse effect level (NOAEL) |
| Authors: | Bhutia, Yangchen Doma
Jain, Neeti
Ahmed, Fakhruddin
Sharma, Manoj
Singh, Ram
Kumar, Satish
Mendki, Murlidhar Jaywantrao
Kumar, Pravin
Vijayaraghavan, R |
| Keywords: | Acute toxicity
Attracticide
Diflubenzuron
Heneicosane
Insect Growth Regulator
NOAEL
Sub-acute toxicity |
| Issue Date: | Jul-2010 |
| Publisher: | CSIR |
| Abstract: | Aedes aegypti mosquito is one of the most
notorious vectors of dangerous diseases like dengue hemorrhagic fever and
chikangunya. One method of control of the vectors is by the use of
semiochemicals or pheromones. The pheromone
n-heneicosane (C21) has been proved to be effective in attracting the female Aedes aegypti to lay eggs in the treated
water and the growth of the larva is controlled by insect growth regulator
diflubenzuron (DB). This study was planned to assess the safety of C21 alone
and the combination with DB. Acute toxicity tests were carried out using two
doses, viz., 1600 and
3200 mg/kg and two routes of exposure oral and intra-peritoneal. Dermal
toxicity test was carried out in both male and female rats at the dose of 3200
mg/kg. Primary skin irritation test was carried out in rabbits. Sub-acute (90
days) dermal toxicity studies in male and female rats at the dose of 1 and 2
mg/kg via the per-cutaneous route were also studied.
Sub-acute (90 days) toxicity test through the oral route was carried out, at
doses 125, 250 and 500 mg/kg in male and female rats. The calculated LD50
by ip route and dermal route was more than 5 g/kg in mouse and rats of both the
sexes. In the primary skin irritation test no significant changes were noted.
In the sub-acute toxicity studies even 500 mg/kg dose was not able to produce
toxic response in rats when they were dosed daily for 90 days. The established no
observed adverse effect level (NOAEL) was more than 500 mg/kg. |
| Page(s): | 744-751 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(07) [July 2010]
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