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Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.11 [2004] >
IJCT Vol.11(6) [November 2004] >
| Title: | Sensitive determination of atenolol in tablets using chloramine-T and two dyes |
| Authors: | Basavaiah, K
Chandrashekar, U
Nagegowda, P |
| Keywords: | Atenolol determination
indigo carmine
metanil yellow
titrimetry
spectrophotometry |
| Issue Date: | Nov-2004 |
| Publisher: | CSIR |
| IPC Code: | GO1J 3/00, GO1N 31/16 |
| Abstract: | Three methods, one
titrimetric and two spectrophotometric, using chloramine-T (CAT) and two dyes,
metanil yellow and indigocarmine have been developed for the determination of
atenolol (ATN) in bulk drug and in tablets. The titrimetric method (Method A)
involves the oxidation of the drug by a measured excess of CAT in acid medium
followed by the determination of the residual oxidant by iodometric back
titration. In spectrophotometric methods, ATN is oxidised by a known excess of
CAT in acid medium followed by the determination of unreacted oxidant by
reacting with a fixed amount of either metanil yellow (Method B) or indigo
carmine (Method C) and measuring the increase in absorbance at 530 or
610 nm. Titrimetry is applicable in 3-20 mg range whereas in
spectrophotometry, Beer's law is obeyed over the concentration ranges, 1-12 μg
mL-1 (Method B) and 2.5 - 20.0 μg mL-1 (Method C). Molar
absorptivity values as calculated from Beer's law data are found to be 1.19×104
L mol-1 cm-1 (Method B) and 6.65×103 L mol -1
cm-1 (Method C) and the corresponding Sandell sensitivity
values are 0.022 and 0.040 μg cm-2. The choice of experimental
conditions, which provide the maximum accuracy, precision and sensitivity, is
discussed. The common excipients and additives found in tablet preparations did
not interfere. The proposed methods were successfully applied to the
determination of the drug in tablets. Recoveries ranged from 96.43 to 103.74 %.
The utility and accuracy of the methods were further established by parallel
determination by the official Indian Pharmacopoeial method and by recovery
studies. |
| Page(s): | 769-776 |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.11(6) [November 2004]
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