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Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.11 [2004] >
IJCT Vol.11(1) [January 2004] >
| Title: | Determination of ranitidine using potassium iodate and dichlorofluorescein |
| Authors: | Basavaiah, K
Nagegowda, P |
| Issue Date: | Jan-2004 |
| Publisher: | CSIR |
| IPC Code: | A61P1/04; G01N31/16; G01N21/25 |
| Abstract: | Two methods for
determining ranitidine hydrochloride (RNH) in pure drug and in formulations
using potassium iodate and dichlorofuorescein are described. Titrimetry
involves the oxidation of RNH by a known excess of potassium iodate in acidic conditions
followed by iodometric determination of surplus iodate. In spectrophotometry
also, the drug is oxidized by a large excess of iodate and the iodine released
is oxidized to ICl2- in
the presence of chloride ions, and is used to iodinate 2,7-dichlorofluorescein
dye and the amount of iodinated dye is measured. Reaction conditions of both
methods have been optimized. In titrimetry, the reaction stoichiometry has been
established and the reaction scheme of the spectrophotometric method is given.
Titrimetry is applicable over 1-16 mg range. In spectrophotometry, the system
obeys Beer’s law for 5-50  g mL-1. The molar absorptivity and Sandell
sensitivity were calculated to be 3.88×103 L Mol-1 cm-1 and 5.72 ng cm-2, respectively. The calculated limits
of detection and quantification were 2.14 and 7.15 g mL-1, respectively. The proposed methods were applied successfully to
the determination of RNH in pharmaceutical preparations with recoveries in the
range of 98.28 ± 0.88 to 103 ± 1.96% (titrimetry) and 99.46 ± 1.88 to 102.58 ± 0.73%
(spectrophotometry). The reliability of the assay was established by parallel
determination by an established procedure and by recovery studies using
standard–addition technique. |
| Page(s): | 11-16 |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.11(1) [January 2004]
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