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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Chemistry -Section B (IJC-B) >
IJC-B Vol.44B [2005] >
IJC-B Vol.44B(12) December 2005] >
| Title: | Design, synthesis and characterisation of a novel anticancer prodrug having antiproliferative activity against prostrate tumour |
| Authors: | Mishra, Satyendra
Tripathi, Snehlata
Mishra, Roli
Misra, Krishna |
| Keywords: | Telomerase
antiproliferative
pro-drug
curcumin-glycine-deoxyoligonucleotide conjugate |
| Issue Date: | Dec-2005 |
| Publisher: | CSIR |
| IPC Code: | Int.Cl.7 C 07 C |
| Abstract: | A curcumin glycine conjugate viz. 1,7-bis (4-O-glycinoyl-3-methoxyphenyl)-1,6-heptadiene-
3, 5 dione 1 previously shown by this lab to be having antioxidant,
antibacterial and antifungal activity is synthesized and after attachment of a
linker (-CH2-CH2-OH) 2 is phosphitylated. The
phosphitylated unit 3 was covalently linked to 5´-GT TAG GGT TAG-3´ 4,
a complementary sequence of 5´-CU AAC CCU AAC-3´, which is the repeat sequence
of telomerase RNA template. The comparative melting temperatures (Tm) of
modified and normal duplexes have indicated more thermal stability of duplex
with bioconjugate. The biodegradable nature of the covalent bond of the prodrug
has now been proved by carrying out enzymatic digestion studies. The prostrate
tumour derived DU145 cells are transfected with 5´-O-curcumin DNA and
study of antiproliferative activity indicated 80% reduction in cell
proliferation by the end of 75 days. |
| Page(s): | 2582-2588 |
| ISSN: | 0975-0983(Online); 0376-4699(Print) |
| Source: | IJC-B Vol.44B(12) December 2005]
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