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Title: Kinetic study on lipase-catalyzed esterification in organic solvents
Authors: Chowdary, G V
Prapulla, S G
Keywords: Eesterification
isovaleric acid
immobilized lipase
<i>Rhizomucor miehei</i>
Issue Date: Nov-2005
Publisher: CSIR
Series/Report no.: <b> Int</b>.<b>Cl</b>.<b><sup>7</sup> C 07 C</b>
Abstract: A twin inhibition is observed for the esterification reaction between ethanol and isovaleric acid using immobilized lipase from <i>Rhizomucor miehei</i> in hexane and in mixed solvent system. The observed bi-substrate inhibition pattern follows a Ping-Pong Bi-Bi mechanism with dead-end inhibition of enzyme by both the substrates. An increase in K<sub>m</sub> value for alcohol in mixed solvent (0.645 <i>M</i>) than in hexane (0.256 <i>M</i>), indicates that the enhanced solvation of ethanol in mixed solvent results in lower degree of inhibition.
Description: 2322-2327
ISSN: 0975-0983(Online); 0376-4699(Print)
Appears in Collections:IJC-B Vol.44B(11) [November 2005]

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