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http://nopr.niscair.res.in/handle/123456789/91
Title: | Kinetic properties and storage stability of catalase immobilized on to florisil |
Authors: | Ozyilmaz, Gul Tukel, S Seyhan Alptekin, Ozlem |
Keywords: | Catalase;Florisil;Covalent immobilization;Storage stability;Relative humidity |
Issue Date: | Feb-2007 |
Publisher: | CSIR |
Abstract: | The covalent immobilization of bovine liver catalase (CAT) on to florisil via glutaraldehyde was investigated. Optimum immobilization pH and temperature were determined as pH 6.0, 10°C respectively, while the amount of initial CAT per g of carrier and immobilization time was determined as 5 mg g⁻¹ and 120 min, respectively. The Vmax values for free and immobilized CAT were found to be 1.7 × 10⁵ and 2.0 × 10⁴ μmol H₂O₂. min⁻¹ mg protein⁻¹, respectively, whereas KM values were 33.3 mM and 1722.0 mM respectively. Operational stability was determined by using a stirred batch-type column reactor. Immobilized CAT retained about 40% of its initial activity after 50 uses. It showed higher storage stability than free CAT at 4°C and 25°C. Its storage stability increased with increasing relative humidity (RH) from 0 to 20% of the medium. The highest storage stability was obtained in 20% RH, however, further increase in RH from 40 to 100% significantly decreased the storage stability. |
Page(s): | 38-43 |
URI: | http://hdl.handle.net/123456789/91 |
ISSN: | 0301-1208 |
Appears in Collections: | IJBB Vol.44(1) [February 2007] |
Files in This Item:
File | Description | Size | Format | |
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IJBB 44(1) (2007) 38-43.pdf | 300.84 kB | Adobe PDF | View/Open |
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