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Title: Kinetic properties and storage stability of catalase immobilized on to florisil
Authors: Ozyilmaz, Gul
Tukel, S Seyhan
Alptekin, Ozlem
Keywords: Catalase;Florisil;Covalent immobilization;Storage stability;Relative humidity
Issue Date: Feb-2007
Publisher: CSIR
Abstract: The covalent immobilization of bovine liver catalase (CAT) on to florisil via glutaraldehyde was investigated. Optimum immobilization pH and temperature were determined as pH 6.0, 10°C respectively, while the amount of initial CAT per g of carrier and immobilization time was determined as 5 mg g⁻¹ and 120 min, respectively. The Vmax values for free and immobilized CAT were found to be 1.7 × 10⁵ and 2.0 × 10⁴ μmol H₂O₂. min⁻¹ mg protein⁻¹, respectively, whereas KM values were 33.3 mM and 1722.0 mM respectively. Operational stability was determined by using a stirred batch-type column reactor. Immobilized CAT retained about 40% of its initial activity after 50 uses. It showed higher storage stability than free CAT at 4°C and 25°C. Its storage stability increased with increasing relative humidity (RH) from 0 to 20% of the medium. The highest storage stability was obtained in 20% RH, however, further increase in RH from 40 to 100% significantly decreased the storage stability.
Page(s): 38-43
ISSN: 0301-1208
Appears in Collections:IJBB Vol.44(1) [February 2007]

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