NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.44 [2007] >
IJBB Vol.44(1) [February 2007] >

Title: Kinetic properties and storage stability of catalase immobilized on to florisil
Authors: Ozyilmaz, Gul
Tukel, S Seyhan
Alptekin, Ozlem
Keywords: Catalase
Covalent immobilization
Storage stability
Relative humidity
Issue Date: Feb-2007
Publisher: CSIR
Abstract: The covalent immobilization of bovine liver catalase (CAT) on to florisil via glutaraldehyde was investigated. Optimum immobilization pH and temperature were determined as pH 6.0, 10°C respectively, while the amount of initial CAT per g of carrier and immobilization time was determined as 5 mg g⁻¹ and 120 min, respectively. The Vmax values for free and immobilized CAT were found to be 1.7 × 10⁵ and 2.0 × 10⁴ μmol H₂O₂. min⁻¹ mg protein⁻¹, respectively, whereas KM values were 33.3 mM and 1722.0 mM respectively. Operational stability was determined by using a stirred batch-type column reactor. Immobilized CAT retained about 40% of its initial activity after 50 uses. It showed higher storage stability than free CAT at 4°C and 25°C. Its storage stability increased with increasing relative humidity (RH) from 0 to 20% of the medium. The highest storage stability was obtained in 20% RH, however, further increase in RH from 40 to 100% significantly decreased the storage stability.
Page(s): 38-43
ISSN: 0301-1208
Source:IJBB Vol.44(1) [February 2007]

Files in This Item:

File Description SizeFormat
IJBB 44(1) (2007) 38-43.pdf300.84 kBAdobe PDFView/Open
 Current Page Visits: 900 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171914 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: