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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(06) [June 2010] >
| Title: | Isolation of genomic DNA from medicinal plants without liquid nitrogen |
| Authors: | Sharma, Pratibha
Joshi, Neha
Sharma, Anubhuti |
| Keywords: | CTAB
DNA isolation
Liquid nitrogen
PCR
RAPD |
| Issue Date: | Jun-2010 |
| Publisher: | CSIR |
| Abstract: | Genomic DNA was extracted from eight
medicinal plants using the present DNA extraction protocols
(CTAB extraction method) with some modifications. Leaves were fixed in
different fixing solutions containing absolute alcohol
(99.99%), chloroform and EDTA, but without liquid nitrogen. DNA
quality and quantity obtained were comparable to those isolated with liquid
nitrogen, as the λ260/λ280 ratio with liquid nitrogen was in range
1.3-1.7 and with other fixing solutions it was 1.1-1.5.
Absolute alcohol showed best results as fixing solution. Good quality of DNA was isolated without using liquid nitrogen from
different medicinal plant species. DNA isolated by this method was suitable for
various molecular biology applications. |
| Page(s): | 610-614 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(06) [June 2010]
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