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IJEB Vol.48(06) [June 2010] >


Title: Isolation of genomic DNA from medicinal plants without liquid nitrogen
Authors: Sharma, Pratibha
Joshi, Neha
Sharma, Anubhuti
Keywords: CTAB
DNA isolation
Liquid nitrogen
PCR
RAPD
Issue Date: Jun-2010
Publisher: CSIR
Abstract: Genomic DNA was extracted from eight medicinal plants using the present DNA extraction protocols (CTAB extraction method) with some modifications. Leaves were fixed in different fixing solutions containing absolute alcohol (99.99%), chloroform and EDTA, but without liquid nitrogen. DNA quality and quantity obtained were comparable to those isolated with liquid nitrogen, as the λ260280 ratio with liquid nitrogen was in range 1.3-1.7 and with other fixing solutions it was 1.1-1.5. Absolute alcohol showed best results as fixing solution. Good quality of DNA was isolated without using liquid nitrogen from different medicinal plant species. DNA isolated by this method was suitable for various molecular biology applications.
Page(s): 610-614
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.48(06) [June 2010]

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