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Title: UV-B induced changes in antioxidant enzymes and their isoforms in cucumber (Cucumis sativus L.) cotyledons
Authors: Kataria, Sunita
Jain, Karishma
Guruprasad, K N
Keywords: Ascorbic acid
Ascorbic acid peroxidase
Cucumber cotyledons
Guaiacol peroxidase
Glutathione reductase
Superoxide dismutase
⍺-Tocopherol
Ultraviolet-B
Issue Date: Feb-2007
Publisher: CSIR
Abstract: To assess the role of antioxidant defense system on exposure to ultra-violet-B (UV-B) radiation, the activities of antioxidant enzymes superoxide dismutase (SOD), ascorbic acid peroxidase (APX), glutathione reductase (GR) and guaiacol peroxidase (GPX), as well as the level of antioxidants ascorbic acid (AA) and ⍺-tocopherol were monitored in cucumber (Cucumis sativus L. var long green) cotyledons. UV-B enhanced the activity of antioxidant enzymes as well as AA content, but decreased the level of ⍺-tocopherol. Significant increase was observed in the activities of SOD and GPX. Analysis of isoforms of antioxidant enzymes by native-PAGE and activity staining revealed three isoforms of GPX in unexposed dark-grown cotyledons (control), and their intensity was enhanced by UV-B exposure. In addition, four new isoforms of GPX were observed in cotyledons after UV-B exposure. Although no new isoforms were observed for the other antioxidant enzymes, the activities of their existing isoforms were enhanced by UV-B.
Page(s): 31-37
ISSN: 0301-1208
Source:IJBB Vol.44(1) [February 2007]

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