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Indian Journal of Chemistry -Section B (IJC-B) >
IJC-B Vol.44B [2005] >
IJC-B Vol.44B(01) [January 2005] >
| Title: | Design, development and synthesis of a novel labeled PNA monomer incorporated in DNA-hexamer to act as a hybridization probe by FRET |
| Authors: | Shukla, Vibha
Mishra, Satyendra
Watal, Geeta
Misra, Krishna |
| Issue Date: | Jan-2005 |
| Publisher: | CSIR |
| IPC Code: | Int.Cl.7 C 07 K |
| Abstract: | A novel PNA monomer with adenine nucleobase and a
modified backbone with charged  and a methylene
substituted for  in the linker
arm has been synthesised. This modified PNA monomer is further linked with
rhodamine via an amide bond, converted to phosphoramidite after linking with a
2-carbon linker. This unit is used for the end coupling at 5'-prime of a hexamer oligonucleotide 5'-TCC ATT-3' in solid phase. The
complementary labeled heptamer has been prepared by coupling the phosphoramidite
of fluorescein with 5'-AAT GGAT-3' as the end coupling step at 5'-prime on solid
phase. The two complementary heptamer oligos are hybridized and the FRET signal
recorded. The Tm of the modified oligomer has been found to be higher as
compared to the normal one. Such a probe can detect single base mismatch by
FRET |
| Page(s): | 121-129 |
| ISSN: | 0975-0983(Online); 0376-4699(Print) |
| Source: | IJC-B Vol.44B(01) [January 2005]
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