NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.44 [2007] >
IJBB Vol.44(1) [February 2007] >


Title: Parallel changes in fibronectin and ⍺₅β₁ integrin in articular cartilage in type II collagen-induced arthritis
Authors: Sandya, S
Achan, M A
Sudhakaran, P R
Keywords: Fibronectin
Integrins
Arthritis
Synovial fluid
Cell-matrix interaction
Issue Date: Feb-2007
Publisher: CSIR
Abstract: Interactions of cells with extracellular matrix (ECM) are mediated through specific cell surface receptors, belonging to the integrin family of transmembrane proteins. Integrins have been shown to be involved in chondrocyte-matrix interactions in the cartilage. In this study, the status of a matrix glycoprotein fibronectin (FN) and its receptor ⍺₅β₁ integrin in the articular cartilage in collagen type II-induced experimental arthritis in rats, as well as in synovial fluid from osteoarthritic patients was investigated. Experimental arthritis was induced by intradermal injection of type-II collagen (300 µg/100 g body wt) and Freund’s complete adjuvant. Saline-treated animals served as control. Clinical severity was indicated by increase in paw volume. Significant increase in the activities of lysosomal enzymes β-glucuronidase and β-hexosaminidase was observed in synovial effusate, serum and cartilage of arthritic animals, when compared to untreated control, indicating dysfunction of cartilage. Changes in FN and ⍺₅β₁ integrin were studied by ELISA. A progressive increase was observed in the FN level in synovial effusate and cartilage of arthritic animals, when compared to untreated controls. FN levels were also significantly high in synovial fluid of osteoarthritic patients. A significant increase in the levels of ⍺₅β₁ integrin was found in cartilage of arthritic rats. Parallel changes in FN and ⍺₅β₁ integrin indicated that alterations in FN and ⍺₅β₁ integrin in chondrocytes constituted one of the molecular mechanisms during progression of arthritis
Page(s): 14-18
ISSN: IJBB Vol.44(1) [February 2007]
Source:IJBB Vol.44(1) [February 2007]

Files in This Item:

File Description SizeFormat
IJBB 44(1) (2007) 14-18.pdf238.25 kBAdobe PDFView/Open
 Current Page Visits: 744 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 557835 since 06-Feb-2009  Last updated on 15-Jul-2014Webmaster: nopr@niscair.res.in