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Title: | Four rapid and sensitive methods for the assay of amoxycillin in pharmaceuticals using bromate-bromide mixture and two dyes |
Authors: | Ramakrishna, V Basavaiah, K |
Keywords: | Amoxycillin determination;titrimetry;spectrophotometry;bromate-bromide;dyes |
Issue Date: | Sep-2005 |
Publisher: | CSIR |
IPC Code: | A61K9/00 |
Abstract: | Four new methods using titrimetry and
spectrophotometry are described for the rapid determination of amoxycillin in
bulk drug and dosage forms. In direct titrimetry (Method A), the drug is
titrated with bromate–bromide mixture in acid medium using methyl orange
indicator. Back titrimetry (Method B) entails adding a known excess amount of
bromate-bromide mixture to the drug solution in acid medium, followed by
determination of residual bromine iodometrically. Both spectrophotometric
methods are based on the oxidation–bromination of amoxyciillin by in situ generated bromine followed by
estimation of unreacted bromine with methyl orange (Method C) or indigo carmine
(Method D) and measuring the change in absorbance at 520 or 610 nm. In all the
methods, qunatitation is based on the amount of bromate that has reacted with
amoxycillin. The experimental conditions have been optimized. Calculations are
based on 1:1 and 1:2 (amoxycillin: bromate) stoichiometry for method A and
Method B, respectively. Method A is applicable over 2-16 mg range, and 1-9 mg
of amoxycillin could be determined by method B. In spectrophotometric methods,
the absorbance is found to increase linearly with increasing concentration of
amoxycillin which is corroborated by the calculated correlation coefficient (r) of 0.9964 (Method C) and 0.9959
(Method D). The calibration graphs are found to be linear over 0.1–1.2 and 0.5 –
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Page(s): | 543-549 |
ISSN: | 0975-0991 (Online); 0971-457X (Print) |
Appears in Collections: | IJCT Vol.12(5) [September 2005] |
Files in This Item:
File | Description | Size | Format | |
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IJCT 12(5) 543-549.pdf | 127.5 kB | Adobe PDF | View/Open |
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