Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/8671
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dc.contributor.authorMinakshi-
dc.contributor.authorPundir, C S-
dc.date.accessioned2010-05-03T06:32:15Z-
dc.date.available2010-05-03T06:32:15Z-
dc.date.issued2005-09-
dc.identifier.issn0975-0991 (Online); 0971-457X (Print)-
dc.identifier.urihttp://hdl.handle.net/123456789/8671-
dc.description539-542en_US
dc.description.abstractLipase, glycerol kinase (GK), glycerol-3-phosphate oxidase and peroxidase were co-immobilized onto alkylamine glass beads affixed on a plastic strip with a conjugation yield of 80.0 mg/g support and 57.41% retention of specific activity. The enzyme showed an increase in optimum pH, incubation temperature, energy of activation and time of incubation, but decrease in Km and Vmax. A method for determination of serum triglycerides was developed, employing strip bound enzymes. The minimum detection limit of the method was 0.61 mM/L. The recovery of added triglycerides was 82.2%. Within and between assay coefficient of variation were < 3.1 and < 14.2 respectively. A good correlation (r=0.973) was found between triglycerides value obtained by commercial enzymatic colourimetric method and present method. The strip provided 100 reuses of co-immobilized enzymes with ease.en_US
dc.language.isoen_USen_US
dc.publisherCSIRen_US
dc.relation.ispartofseriesA61K38/43en_US
dc.sourceIJCT Vol.12(5) [September 2005]en_US
dc.subjectTriglyceridesen_US
dc.subjectlipaseen_US
dc.subjectGKen_US
dc.subjectGPOen_US
dc.subjectperoxidaseen_US
dc.subjectalkylamine glass beaden_US
dc.subjectplastic stripen_US
dc.subjectco-immobilizationen_US
dc.titlePreparation of reusable enzyme strip for determination of serum triglycerideen_US
dc.typeArticleen_US
Appears in Collections:IJCT Vol.12(5) [September 2005]

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