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Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.12 [2005] >
IJCT Vol.12(5) [September 2005] >
| Title: | Preparation of reusable enzyme strip for determination of serum triglyceride |
| Authors: | Minakshi
Pundir, C S |
| Keywords: | Triglycerides
lipase
GK
GPO
peroxidase
alkylamine glass bead
plastic strip
co-immobilization |
| Issue Date: | Sep-2005 |
| Publisher: | CSIR |
| IPC Code: | A61K38/43 |
| Abstract: | Lipase, glycerol kinase (GK),
glycerol-3-phosphate oxidase and peroxidase were co-immobilized onto alkylamine
glass beads affixed on a plastic strip with a conjugation yield of 80.0 mg/g
support and 57.41% retention of specific activity. The enzyme showed an
increase in optimum pH, incubation temperature, energy of activation and time
of incubation, but decrease in Km
and Vmax. A method
for determination of serum triglycerides was developed, employing strip bound
enzymes. The minimum detection limit of the method was 0.61 mM/L. The recovery
of added triglycerides was 82.2%. Within and between assay coefficient of
variation were < 3.1 and < 14.2 respectively. A good correlation (r=0.973) was found between triglycerides
value obtained by commercial enzymatic colourimetric method and present method.
The strip provided 100 reuses of co-immobilized enzymes with ease. |
| Page(s): | 539-542 |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.12(5) [September 2005]
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