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Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.12 [2005] >
IJCT Vol.12(3) [May 2005] >
| Title: | Determination of tinidazole by potentiometry, spectrophotometry and high performance liquid chromatography |
| Authors: | Basavaiah, K
Nagegowda, P
Chandrashekar, U |
| Keywords: | Tinidazole
potentiometry
spectrophotometry
HPLC
pharmaceuticals |
| Issue Date: | May-2005 |
| Publisher: | CSIR |
| IPC Code: | G01J3/00; B01D15/00; A61K |
| Abstract: | Three assay procedures based on
potentiometry, spectrophotometry and high performance liquid chromatography
(HPLC) have been developed for the determination of tinidazole in tablets and
injections. In the potentiometric titration method, the drug in glacial acetic
acid is titrated with acetous perchloric acid with potentiometric end point
detection. Spectrophotometry involves the addition of different amounts of
tinidazole to a fixed amount of perchloric acid-malachite green mixture
followed by measurement of absorbance at 615 nm. The HPLC determination was
carried out on a reversed phase C18 column using a mobile phase
consisting of acetonitrile-0.1 % phosphoric acid (70:30) at a flow rate of 1.0
mL min-1 with UV-detection at 316 nm. Potentiometric tiration is
applicable over 1-10 mg range of tinidazole and in spectrophotometry, the
calibration graph is linear from 15-180 mg mL-1 with a molar absorptivity of 9.02  102 L moL-1 cm-1 and a Sandell sensitivity
of 385.2 ng cm-2. The limits of detection and quantification are
calculated to be 3.05 and 10.15  g mL-1,
respectively. In HPLC method, a rectilinear relationship was observed between
6.25 and 250 g mL-1 tinidazole with a
detection limit of 0.625 g mL-1
and a quantification limit of 1.875 g mL-1.
The analysis time was less than 5 min. The statistical evaluation of the
methods was examined by determining intra-day and inter-day precision. The
methods when applied to the determination of tinidazole in tablets and
injections gave satisfactory results. The accuracy and reliability of the
proposed methods were further ascertained by parallel determination by the
reference methods and by recovery studies using standard-addition technique. |
| Page(s): | 273-280 |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.12(3) [May 2005]
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