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Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.12 [2005] >
IJCT Vol.12(2) [March 2005] >
| Title: | Assay of bromhexine hydrochloride in pharmaceutical formulations by extraction spectrophotometry |
| Authors: | Rao, S V Murali Mohan
Rao, I Nageswara
Reddy, T Rama Subba
Sastry, C S P |
| Keywords: | Spectrophotometry
tropaeolin oo
naphthalene blue 12BR
azocarmine G |
| Issue Date: | Mar-2005 |
| Publisher: | CSIR |
| IPC Code: | G01J 3/00; A61K 9/00 |
| Abstract: | Three simple and sensitive
spectrophotometric methods (A-C) for the assay of bromhexine hydrochloride in
pure and dosage forms based on the formation of chloroform soluble
ion-associates under specified experimental conditions are described. Three
acidic dyes, namely, Tropaeolin oo (TP oo, method A), Naphthalene blue 12BR (NB
12BR, method B) and Azocarmine G (ACG, method C) are utilized. The extracts of
the ion-associates exhibit absorption maxima at 420, 620 and 540 nm for methods
A, B and C respectively. Regression analysis of Beer-Lambert plots showed good
correlation in the concentration ranges (2.0-10)  g/mL for method A, (5.0-25) g/mL for methods B and C respectively. Beer’s law and the precision
and accuracy of the methods are checked by the UV reference method. The results
are reproducible with an accuracy of ± 1.0%. The methods are found to be
suitable for the determination of bromhexine hydrochloride in the presence of
the other ingredients that are usually present in dosage forms. |
| Page(s): | 170-174 |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.12(2) [March 2005]
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