|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.12 [2005] >
IJCT Vol.12(2) [March 2005] >
| Title: | Rapid titrimetric and spectrophotometric determination of frusemide (furosemide) in formulations using bromate-bromide mixture and methyl orange |
| Authors: | Basavaiah, K
Chandrashekar, U
Nagegowda, P |
| Keywords: | Frusemide
determination
titrimetry
spectrophotometry
bromate-bromide
methyl orange |
| Issue Date: | Mar-2005 |
| Publisher: | CSIR |
| IPC Code: | G01J3/00; A61K |
| Abstract: | Two methods based on titrimetric and
spectrophotometric techniques using bromate-bromide mixture and methyl orange
as reagents are described for the determination of frusemide in bulk drug and
formulations. In titrimetry, an acidified solution of frusemide is titrated
directly with bromate-bromide mixture using methyl orange as indicator.
Spectrophotometry entails adding a fixed and known amount of bromate-bromide
mixture to an acidified solution of drug and determination of the residual
bromine by reacting with a fixed amount of methyl orange and measuring the
absorbance at 520 nm. The amount of bromine reacted corresponds to drug
content. The quantification in titrimetry is based on a 1:0.333 reaction
stoichiometry (Frusemide: KBrO3) and the method is applicable over
2-20 mg range. In spectrophotometry, the calibration graph is found to be
linear over 0.25-3.50 µg mL-1 range with a molar absorptivity of
6.11 104 L mol-1 cm-1
and a Sandell sensitivity of 5.18 ng cm-2. The limits of detection
and quantification are calculated to be 0.07 µg mL-1 and 0.24 µg mL-1,
respectively. The statistical evaluation of the methods was examined by
determining the intra-day and inter-day precision. The methods were applied to
the determination of frusemide in tablets and the results were found to agree
well with the declared amounts. The accuracy and reliability of the proposed
methods were further ascertained by parallel determination by a reference
method and by recovery studies using standard addition technique. |
| Page(s): | 149-155 |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.12(2) [March 2005]
|
|
