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dc.contributor.authorNarayana, B-
dc.contributor.authorDivya, N S-
dc.identifier.issn0975-1084 (Online); 0022-4456 (Print)-
dc.description.abstractA facile spectrophotometric method is proposed for determination of Colchicoside (COLSID) in bulk and in dosage forms using ceric ammonium sulphate (CAS). Method involves addition of a known excess of CAS to COLSID and determination of residual CAS with a fixed amount of either Azure B (AB), measuring absorbance at 633nm or Safranin O (SO), measuring absorbance at 519 nm. Calibration graphs are linear over 25.00-50.00 μgml-1, 22.00-38.00 μgml-1 and apparent molar absorptivity is calculated to be 2.28 x 104 l mol-1 cm-1 and 2.77 x 104 l mol-1 cm-1 for CAS-AB and CAS-SO respectively. Method is successfully applied to assay of COLSID in pharmaceutical formulations.en_US
dc.sourceJSIR Vol.69(05) [May 2010]en_US
dc.subjectAzure B (AB)en_US
dc.subjectCeric ammonium sulphate (CAS)en_US
dc.subjectColchicoside (COLSID)en_US
dc.subjectSafranin O (SO)en_US
dc.titleA new method for spectrophotometric determination of colchicosideen_US
Appears in Collections:JSIR Vol.69(05) [May 2010]

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