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Title: A new method for spectrophotometric determination of colchicoside
Authors: Narayana, B
Divya, N S
Keywords: Azure B (AB)
Ceric ammonium sulphate (CAS)
Colchicoside (COLSID)
Safranin O (SO)
Issue Date: May-2010
Publisher: CSIR
Abstract: A facile spectrophotometric method is proposed for determination of Colchicoside (COLSID) in bulk and in dosage forms using ceric ammonium sulphate (CAS). Method involves addition of a known excess of CAS to COLSID and determination of residual CAS with a fixed amount of either Azure B (AB), measuring absorbance at 633nm or Safranin O (SO), measuring absorbance at 519 nm. Calibration graphs are linear over 25.00-50.00 μgml-1, 22.00-38.00 μgml-1 and apparent molar absorptivity is calculated to be 2.28 x 104 l mol-1 cm-1 and 2.77 x 104 l mol-1 cm-1 for CAS-AB and CAS-SO respectively. Method is successfully applied to assay of COLSID in pharmaceutical formulations.
Page(s): 368-372
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.69(05) [May 2010]

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