|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.69 [2010] >
JSIR Vol.69(05) [May 2010] >
| Title: | A new method for spectrophotometric determination of colchicoside |
| Authors: | Narayana, B
Divya, N S |
| Keywords: | Azure B (AB)
Ceric ammonium sulphate (CAS)
Colchicoside (COLSID)
Safranin O (SO) |
| Issue Date: | May-2010 |
| Publisher: | CSIR |
| Abstract: | A facile spectrophotometric method is proposed for determination of Colchicoside (COLSID) in bulk and in dosage forms using ceric ammonium sulphate (CAS). Method involves addition of a known excess of CAS to COLSID and determination of residual CAS with a fixed amount of either Azure B (AB), measuring absorbance at 633nm or Safranin O (SO), measuring absorbance at 519 nm. Calibration graphs are linear over 25.00-50.00 μgml-1, 22.00-38.00 μgml-1 and apparent molar absorptivity is calculated to be 2.28 x 104 l mol-1 cm-1 and 2.77 x 104 l mol-1 cm-1 for CAS-AB and CAS-SO respectively. Method is successfully applied to assay of COLSID in pharmaceutical formulations. |
| Page(s): | 368-372 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.69(05) [May 2010]
|
|
