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|Title:||A new method for spectrophotometric determination of colchicoside|
Divya, N S
|Keywords:||Azure B (AB);Ceric ammonium sulphate (CAS);Colchicoside (COLSID);Safranin O (SO)|
|Abstract:||A facile spectrophotometric method is proposed for determination of Colchicoside (COLSID) in bulk and in dosage forms using ceric ammonium sulphate (CAS). Method involves addition of a known excess of CAS to COLSID and determination of residual CAS with a fixed amount of either Azure B (AB), measuring absorbance at 633nm or Safranin O (SO), measuring absorbance at 519 nm. Calibration graphs are linear over 25.00-50.00 μgml-1, 22.00-38.00 μgml-1 and apparent molar absorptivity is calculated to be 2.28 x 104 l mol-1 cm-1 and 2.77 x 104 l mol-1 cm-1 for CAS-AB and CAS-SO respectively. Method is successfully applied to assay of COLSID in pharmaceutical formulations.|
|ISSN:||0975-1084 (Online); 0022-4456 (Print)|
|Appears in Collections:||JSIR Vol.69(05) [May 2010]|
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