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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(05) [May 2010] >
| Title: | Cysteine rich cyanopeptide  2 from Spirulina fussiformis exhibits plasmid DNA pBR322 scission prevention and cellular antioxidant activity |
| Authors: | Madhyastha, H
Vatsala, T M |
| Keywords: | Antioxidation
C-phycocyanin peptide
DNA scission activity
Spirulina fussiformis |
| Issue Date: | May-2010 |
| Publisher: | CSIR |
| Abstract: |
Isolation of
three different active peptides from C-phycocyanin (C-pc) b chain of
S. fussiformis
and their biological properties are reported. Phycocyanin peptide β fraction 2 (cyanopeptide β 2) facilitated both
antioxidant and plasmid DNA strand scission prevention activity due to higher
cysteine moieties in the isolated peptide. The peptide significantly scavenged
the free radicals like 1-1,-diphenyl-2-picryl hydrazyl and ferric reducing
ability of plasma, increased the absorbance values in reducing power and also
showed the higher trolox equivalent antioxidant capacity values in total reactive antioxidant potentials assay. Cyanopeptide
b 2 also inhibited
reactive oxygen species induced DNA pBR322 damage in dose dependent manner along
with free radical scavenging properties suggesting the role in the DNA
integrity which is also evident by DNA binding activity of peptide. In
addition, the generation of reactive oxygen species (ROS) was dose dependent (10
and 20 ng/ml) and significantly quenched by cyanopeptide b2 in human fibroblast cell line TIG 3-20. In vitro cell scratch injury assay
demonstrated the capacity of cyanopeptide b2 in cell migration in to wounded area suggesting fibroblast proliferation
and migration. The results suggest that cyanopeptide b2 can be a free radical scavenger and effective peptide for future
biomedical applications like wound healing, atherosclerosis, cell redox
potential and hypoxia.
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| Page(s): | 486-493 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(05) [May 2010]
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