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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(05) [May 2010] >
| Title: | Noggin induces human bone marrow-derived mesenchymal stem cells to differentiate into neural and photoreceptor cells |
| Authors: | Tao, Yu X.
Xu, Hai W.
Yin, Zheng Q.
Gibbon, Thomas Fitz |
| Keywords: | Epidermal growth factor
Mesenchymal stem cells
Noggin
Photoreceptor
Trans-differentiation |
| Issue Date: | May-2010 |
| Publisher: | CSIR |
| Abstract: | The
present study was undertaken to explore the effect of noggin on neuronal
differentiating potential of human bone
marrow-derived mesenchymal stem cells (hBMMSCs) in vitro so as to provide a means of alleviate retinal
degeneration. A green fluorescent protein-tagged noggin gene was
transferred into adult hBMMSCs or induce hBMMSCs with classical inducer, epidermal growth factor(EGF). Neurons were observed as early as 48 h after transduction
of hBMMSCs with a noggin adenoviral
vector. Differentiation peaked by 10 days in culture, and these differentiated cells
expressed multiple markers including rhodopsin (18.4 ± 1.5% of cells), chx10
(4.8 ± 0.6%), nestin (4.2 ± 0.8%), and Nrl (3.7 ± 0.4%), as verified by
immunofluorescence staining. Noggin-transduced cells produced more
photoreceptor cells than non-transduced cells, suggesting that noggin has the
ability to induce hBMMSCs to trans-differentiate into photoreceptor cells. In
contrast, induction with EGF for 10 days led to lower levels of rhodopsin and chx10,
and undetectable levels of Nrl and Nestin.
These findings suggested
noggin-transduced hBMMSCs produced more photoreceptor cells than EGF–induced cells. It is suggested that the
present protocol has application in cell replacement therapy for patients suffering
from photoreceptor cell loss. |
| Page(s): | 444-452 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(05) [May 2010]
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