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Title: Noggin induces human bone marrow-derived mesenchymal stem cells to differentiate into neural and photoreceptor cells
Authors: Tao, Yu X.
Xu, Hai W.
Yin, Zheng Q.
Gibbon, Thomas Fitz
Keywords: Epidermal growth factor
Mesenchymal stem cells
Issue Date: May-2010
Publisher: CSIR
Abstract: The present study was undertaken to explore the effect of noggin on neuronal differentiating potential of human bone marrow-derived mesenchymal stem cells (hBMMSCs) <i style="">in vitro</i> so as to provide a means of alleviate retinal degeneration. A green fluorescent protein-tagged noggin gene was transferred into adult hBMMSCs or induce hBMMSCs with classical inducer, epidermal growth factor(EGF). Neurons were observed as early as 48 h after transduction of hBMMSCs with a noggin adenoviral vector. Differentiation peaked by 10 days in culture, and these differentiated cells expressed multiple markers including rhodopsin (18.4 ± 1.5% of cells), chx10 (4.8 ± 0.6%), nestin (4.2 ± 0.8%), and Nrl (3.7 ± 0.4%), as verified by immunofluorescence staining. Noggin-transduced cells produced more photoreceptor cells than non-transduced cells, suggesting that noggin has the ability to induce hBMMSCs to trans-differentiate into photoreceptor cells. In contrast, induction with EGF for 10 days led to lower levels of rhodopsin and chx10, and undetectable levels of Nrl and Nestin.<b style=""> </b>These findings suggested noggin-transduced hBMMSCs produced more photoreceptor cells than EGF–induced cells. It is suggested that the present protocol has application in cell replacement therapy for patients suffering from photoreceptor cell loss.
Description: 444-452
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.48(05) [May 2010]

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