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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(05) [May 2010] >
| Title: | A novel DNA vaccine constructed by heat shock protein 70 and melanoma antigen-encoding gene 3 against tumorigenesis |
| Authors: | Qu, Ping
Ma, Jia-Hai
Zhang, Xiu-Min
Huang, Xiao-Jun
Yang, Xin-Wei
Sui, Yan-Fang |
| Keywords: | Antitumor agents
Heat-shock proteins 70
MAGE-3 protein
Recombinant DNA vaccines |
| Issue Date: | May-2010 |
| Publisher: | CSIR |
| Abstract: | Melanoma antigen-encoding gene 3 (MAGE-3) is an ideal candidate for a tumor vaccine although its potency need
to be increased. Heat shock
proteins (HSPs) represents a potential approach for increasing the
potency of DNA vaccines. In
the present study, a fusion DNA vaccine composed of Mycobacterium tuberculosis HSP70 and MAGE-3 was constructed and
used to immunize C57BL/6 mice against B16 or B16-MAGE-3 tumor cells. The
results show that the HSP70-MAGE-3 fusion DNA vaccine enhanced the frequency of MAGE-3-specific cytotoxic T-cells as
compared to the MAGE-3 DNA vaccine or the HSP70/MAGE-3 cocktail DNA vaccine (P<0.05). In conclusion, the results
indicate that the HSP70-MAGE-3 fusion
DNA vaccine can strongly activate MAGE-3 specific cellular immunological reactions and thus significantly inhibit the growth of
B16-MAGE-3 tumors, improving the survival of tumor-bearing mice, and the HSP70-MAGE-3 fusion DNA vaccine has
a significant therapeutic effect on the tumors that express MAGE-3 antigens. |
| Page(s): | 436-443 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(05) [May 2010]
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