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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(2) [April 2010] >
| Title: | Assessment of free radical-mediated damage in head and neck squamous cell carcinoma patients and after treatment with radiotherapy |
| Authors: | Gupta, Aashita
Bhatt, M L B
Misra, M K |
| Keywords: | Head and neck cancer
Oxidative stress
Superoxide dismutase
Catalase
Total thiols
Malondialdehyde
Reactive oxygen species |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: | Reactive oxygen species (ROS) produced as a part of cellular metabolism
can interact with biological macromolecules such as DNA, proteins and lipids
and interfere with their normal functions, leading to the loss of cellular
viability. ROS have been implicated in many pathophysiological conditions
including cancer. In the present study, the damage caused by ROS and the effect
of radiation in head and neck squamous cell carcinoma (HNSCC) patients were
assessed in the erythrocytes by analyzing the superoxide dismutase (SOD) and
catalase (CAT) activities, and levels of total thiols (T-SH) and
malondialdehyde (MDA, a marker for lipid peroxidation). Blood samples were
collected before the start of treatment and after the completion of
radiotherapy. Both SOD and CAT activities were decreased in untreated patients,
but elevated in patients after treatment. The
T-SH levels were also depleted in untreated HNSCC patients, but elevated
non-significantly after radiation therapy (p>0.05). The levels of MDA showed
a significant increase in both untreated patients and after radiation therapy
when compared with normal subjects (p<0.05). Thus, the present study
indicated that the free radical-mediated damage was aggravated in untreated
HNSCC patients, but the levels of antioxidants returned to baseline or nearly
so after the treatment with radiation therapy. |
| Page(s): | 96-99 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(2) [April 2010]
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