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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(2) [April 2010] >
| Title: | Hsp90 mediates activation of the heme regulated eIF-2 kinase during oxidative stress |
| Authors: | Kulkarni, A P
Mittal, S P
Devasagayam, T P A
Pal, J K |
| Keywords: | eIF-2 Kinase
Hsp70
Hsp90
Protein synthesis
Oxidative stress |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: | The heme-regulated
inhibitor (HRI), a member of the eIF-2 kinase family
is crucial for regulating protein synthesis during stress. In addition to heme,
stress proteins Hsp90 and Hsp70 are known to regulate HRI. The present study
aims to determine the physical association of these Hsps in the regulation of
HRI activation during oxidative stress using human K562 cells as a model.
Extracts from the stress-induced cells were used for determining HRI kinase
activity by measuring eIF-2
phosphorylation, and Hsp-HRI interaction by immunoprecipitation and immunoblot
analyses. The results indicate a significant increase in both Hsp70 and Hsp90
expression during AAPH (2, 2’-azobis (2-amidinopropane) dihydrochloride)-induced
oxidative stress. Further, their interaction with HRI, which correlates well
with its increased HRI kinase activity leads to inhibition of protein
synthesis. Thus, we demonstrate that Hsps play an important role in the
regulation of initiation of protein synthesis during oxidative stress. |
| Page(s): | 67-74 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(2) [April 2010]
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