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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(2) [April 2010] >
| Title: | Antioxidant activity of acetone extract/fractions of Terminalia bellerica Roxb. fruit |
| Authors: | Guleria, Sanjay
Tiku, A K
Rana, Subhash |
| Keywords: | Antioxidant activity
Flavonoids
Free radical
Phenols
Terminalia bellerica |
| Issue Date: | Apr-2010 |
| Publisher: | CSIR |
| Abstract: | Terminalia bellerica Roxb. (Family: Combretaceae) has been valued in Indian system of
medicine for treatment of wide range of diseases and reported to have
antioxidant properties. In the present study, the free radical scavenging
activity and antioxidant potential of acetone extract/fractions of its fruit
was investigated using in vitro assays, including scavenging
ability against 2,2′-diphenyl-2-picrylhydrazyl (DPPH), β-carotene bleaching
inhibition, reducing power and chelating ability on Fe2+ ions. The
fruit powder was extracted at room temperature with different solvents in the
order of increasing and decreasing polarity to obtain crude acetone extract
which was further partitioned with ethyl acetate and water (1:1). It was found
that ethyl acetate fraction was more effective than crude acetone extract in
all antioxidant assays, except chelating power which was highest in water fraction.
Maximum antioxidant activities (expressed as EC50 values) observed
were
14.56 μg/ml, 27.81 μg/ml and 67.8 μg/ml in DPPH, β-carotene bleaching and
reducing power assays, respectively. The antioxidant potential was compared
with known antioxidant (butylated hydroxyl toluene) and correlated with total
phenolic and flavonoid content in crude extract and fractions. Fractions rich
in polyphenolic content were more effective than the crude extract. |
| Page(s): | 110-116 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(2) [April 2010]
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