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|Title: ||Prophylactic treatment with Bacopa monnieri leaf powder mitigates paraquat- induced oxidative perturbations and lethality in Drosophila melanogaster|
|Authors: ||Hosamani, Ravikumar|
|Keywords: ||Paraquat;Oxidative stress;Mitochondrial dysfunction;Bacopa monnieri;Neuroprotection|
|Issue Date: ||Apr-2010|
Environmental exposure to the
oxidant-producing herbicide, paraquat (PQ) (1, 1’-dimethyl-4, 4’-bipyridinium
dichloride) has long been implicated as a risk factor in Parkinson’s disease
(PD). PQ-induced oxidative stress has been exploited as a model to screen
putative neuroprotective compounds employing Drosophila. In the present study, we investigated the prophylactic
efficacy of Bacopa monnieri (BM)
against PQ-induced oxidative stress, mitochondrial dysfunctions and lethality.
Exposure of adult male flies (Oregon K) to PQ alone (40 mM in 5% sucrose) resulted
in 50% mortality at 48 h. Prophylaxis (7 days) with BM extract (0.1%) offered
significant protection (40%) against PQ-induced mortality. Further, oxidative
impairments and mitochondrial dysfunctions were monitored among Drosophila exposed to PQ (20, 40 mM) for 24 h. Significant
induction of oxidative stress was observed in terms of enhanced malondialdehyde
and hydroperoxide levels, and elevated activities of antioxidant enzymes
(catalase and SOD). Mitochondrial dysfunctions included of significant
reduction in the activities of succinate dehydrogenase (23%), complex I-III
(26%), and complex II-III (30%) enzymes. Interestingly, prophylaxis with BM
extract prevented the oxidative stress induction by PQ and restored the
activity of ETC complexes, suggesting clearly its specific effect on the mitochondria.
While the precise mechanism of action of BM needs further investigations, it
may be related to its ability to enhance antioxidant defences and thus mitigate
PQ-induced oxidative stress in Drosophila.
|ISSN: ||0975-0959 (Online); 0301-1208 (Print)|
|Appears in Collections:||IJBB Vol.47(2) [April 2010]|
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