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|Abstract:||The scientists at Russian Academy of Agricultural Sciences, Moscow, Russia have isolated a 30-residue antimicrobial peptide Ar-AMP from the seeds of amaranth, <b style=""><i>Amaranthus retroflexus</i> Linn.</b> essentially by a single step procedure using reversed-phase HPLC, and its <i style="">in vitro</i> biological activities were studied. The complete amino acid sequence of Ar-AMP was determined by Edman degradation in combination with mass spectrometric methods. In addition, the cDNA encoding Ar-AMP was obtained and sequenced. The cDNA encodes a precursor protein consisting of the N-terminal putative signal sequence of 25 amino acids, a mature peptide of 30 amino acids and a 34-residue long C-terminal region cleaved during post-translational processing. According to sequence similarity the Ar-AMP belongs to the hevein-like family of antimicrobial peptides with six cysteine residues. In spite of the fact that seeds were collected in 1967 and lost their germination capacity, Ar-AMP retained its biological activities. It effectively inhibited the growth of different fungi tested: <i>Fusarium culmorium</i> (Smith) Sacc., <i>Helminthosporium sativum</i>, <i>Alternaria consortiale</i> Fr., and <i>Botrytis cinerea</i> Pers. caused morphological changes in <i>Rhizoctonia solani</i> Kühn at micromolar concentrations and protected barley seedlings from <i>H. sativum</i> infection [Lipkin Aleksey, Anisimova Veronika, Nikonorova Aleksandra, Babakov Aleksey Aleksey, Krause Eberhardt, Bienert Mikhael, Grishin Eugene <sup> </sup>and Egorov Tsezi, An antimicrobial peptide Ar-AMP from amaranth (<i>Amaranthus retroflexus</i> L.) seeds, <i style="">Phytochemistry</i>, 2005, <b style="">66</b>(20), 2426-2431]. <b style=""><span style="color: rgb(153, 51, 0); font-size: 14pt;"></span></b>|
|ISSN:||0975-1092 (Online); 0972-592X (Print)|
|Appears in Collections:||NPR Vol.4(6) [November-December 2005]|
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