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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
NPR Vol.4 [2005] >
NPR Vol.4(6) [November-December 2005] >
| Title: | Insecticide/Fungicide |
| Issue Date: | Dec-2005 |
| Publisher: | CSIR |
| Abstract: | The
scientists at Russian Academy of Agricultural Sciences, Moscow, Russia have
isolated a 30-residue antimicrobial peptide Ar-AMP from the seeds of amaranth,
Amaranthus retroflexus Linn.
essentially by a single step procedure using reversed-phase HPLC, and its in vitro biological activities were
studied. The complete amino acid sequence of Ar-AMP was determined by Edman
degradation in combination with mass spectrometric methods. In addition, the
cDNA encoding Ar-AMP was obtained and sequenced. The cDNA encodes a precursor
protein consisting of the N-terminal putative signal sequence of 25 amino acids,
a mature peptide of 30 amino acids and a 34-residue long C-terminal region
cleaved during post-translational processing. According to sequence similarity
the Ar-AMP belongs to the hevein-like family of antimicrobial peptides with six
cysteine residues. In spite of the fact that seeds were collected in 1967 and
lost their germination capacity, Ar-AMP retained its biological activities. It
effectively inhibited the growth of different fungi tested: Fusarium
culmorium (Smith) Sacc., Helminthosporium sativum, Alternaria
consortiale Fr., and Botrytis cinerea Pers. caused morphological
changes in Rhizoctonia solani Kühn at micromolar concentrations and
protected barley seedlings from H. sativum infection [Lipkin Aleksey, Anisimova Veronika, Nikonorova Aleksandra, Babakov Aleksey Aleksey, Krause Eberhardt,
Bienert Mikhael, Grishin Eugene and Egorov Tsezi, An antimicrobial
peptide Ar-AMP from amaranth (Amaranthus retroflexus L.) seeds, Phytochemistry, 2005, 66(20), 2426-2431].
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| Page(s): | 508 |
| ISSN: | 0975-1092 (Online); 0972-592X (Print) |
| Source: | NPR Vol.4(6) [November-December 2005]
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