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|Abstract:||The scientists at Russian Academy of Agricultural Sciences, Moscow, Russia have isolated a 30-residue antimicrobial peptide Ar-AMP from the seeds of amaranth, Amaranthus retroflexus Linn. essentially by a single step procedure using reversed-phase HPLC, and its in vitro biological activities were studied. The complete amino acid sequence of Ar-AMP was determined by Edman degradation in combination with mass spectrometric methods. In addition, the cDNA encoding Ar-AMP was obtained and sequenced. The cDNA encodes a precursor protein consisting of the N-terminal putative signal sequence of 25 amino acids, a mature peptide of 30 amino acids and a 34-residue long C-terminal region cleaved during post-translational processing. According to sequence similarity the Ar-AMP belongs to the hevein-like family of antimicrobial peptides with six cysteine residues. In spite of the fact that seeds were collected in 1967 and lost their germination capacity, Ar-AMP retained its biological activities. It effectively inhibited the growth of different fungi tested: Fusarium culmorium (Smith) Sacc., Helminthosporium sativum, Alternaria consortiale Fr., and Botrytis cinerea Pers. caused morphological changes in Rhizoctonia solani Kühn at micromolar concentrations and protected barley seedlings from H. sativum infection [Lipkin Aleksey, Anisimova Veronika, Nikonorova Aleksandra, Babakov Aleksey Aleksey, Krause Eberhardt, Bienert Mikhael, Grishin Eugene and Egorov Tsezi, An antimicrobial peptide Ar-AMP from amaranth (Amaranthus retroflexus L.) seeds, Phytochemistry, 2005, 66(20), 2426-2431].|
|ISSN:||0975-1092 (Online); 0972-592X (Print)|
|Appears in Collections:||NPR Vol.4(6) [November-December 2005]|
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